Accession Number : ADA133526

Title :   Effect of Radiofrequency Radiation on DNA Duplex Stability and Replication.

Descriptive Note : Final rept. 30 Apr 81-30 Nov 82,

Corporate Author : MISSOURI UNIV-ROLLA

Personal Author(s) : Brown,Roger F ; Marshall,Stanley V

Full Text :

Report Date : Aug 1983

Pagination or Media Count : 45

Abstract : Three experimental approaches were used to determine if absorption of continuous wave radiofrequency (RF) photons affect the stability and/or replication of mammalian DNA. Two of the approaches involved experiments with female CD-1 mice, including analyses of RF effects on sister chromatid exchanges (SCE) in bone marrow as one index of DNA stability and RF effects on replication of the animals' marrow and spleen DNA. The third experimental approach consisted of tests to determine if RF exposure causes partial denaturation of double-stranded DNA, monitored in this study by susceptibility of the polymer to hydrolysis by single-strand-specific Sl nuclease. The substrate DNA used for this cell-free assay was isolated from Chinese hamster ovary cell cultures labeled with radioactive deoxynucleosides. Irradiated subjects were exposed to incident RF field densities adjusted to be equivalent to absorbed doses in mice of 4 W/kg at each of three test frequencies: 400, 800, and 1200 MHz. The results revealed no reduction in the level of DNA synthesis in either the spleen or the bone marrow of animals exposed to any of the three test frequencies, also no increase in the number of SCE. However, a slight, but consistent, increase in the nuclease susceptibility of isolated DNA appeared to be a result of RFR exposure.

Descriptors :   *Radiation effects , *Deoxyribonucleic acids , *Radiofrequency , Stability , Bone marrow , Hydrolysis , Mice , Instrumentation , Density , Measurement , Biosynthesis , Laboratory equipment , Chambers , Test equipment , Anechoic chambers , Nuclease

Subject Categories : Radiobiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE