Accession Number : ADA132481

Title :   The Effects of Various Levels of Ascorbic Acid Intake Upon Oral Wound Healing in Guinea Pigs.

Descriptive Note : Master's thesis,


Personal Author(s) : Stanya,Andrew E

Full Text :

Report Date : Jan 1983

Pagination or Media Count : 109

Abstract : The purpose of this study was to evaluate the effects of varying levels of orally administered ascorbic acid on wound healing in guinea pig oral mucosa. Forty five Murphy/Hartley guinea pigs were randomly placed into four groups and fed an ascorbic acid deficient diet for 2 weeks. Each group of animals then received a daily oral supplement of the following doses of ascorbic acid: 0.5 mg., 5.0 mg., 50 mg., and 250 mg. All animals were weighed twice a week. Twenty eight days later, a standardized wound was made in the premaxilla. On day 36, all animals were sacrificed. Blood samples were evaluated for levels of ascorbic acid. Thirty nine samples showed insignificant levels of vitamin C, 32 of which showed no ascorbic acid. Block sections of the premaxillary wound site were processed for histologic evaluation. Cross sections of the healing wounds were stained with H+E and Masson and were evaluated using a quadratic test grid. Results revealed that varying the levels of ascorbic acid had no significant effect on wound healing at 8 days (P.05).

Descriptors :   *Ascorbic acid , *Healing , Physiological effects , Wounds and injuries , Guinea pigs , Oral dosage , Blood analysis , Histology , Organs(Anatomy) , Body weight , Level(Quantity)

Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE