Accession Number : ADA130518
Title : Pathogenesis of Dengue Vaccine Viruses in Mosquitoes.
Descriptive Note : Annual progress rept. no. 1, 1 Jul 79-1 Jan 80,
Corporate Author : YALE UNIV NEW HAVEN CONN SCHOOL OF MEDICINE
Personal Author(s) : Beaty,Barry J ; Aitken,Thomas H G
Report Date : 01 Jan 1980
Pagination or Media Count : 23
Abstract : An in vitro assay was developed to detect transmission of Dengue-2 parent and vaccine virus strains by vector mosquitoes. After interthoracic inoculations, transmission of both viruses was detected at 7 and 14 days incubation. Rates of transmission were similar. A mosquito inoculation-headsquash-immunofluorescence technique was found to be more sensitive than Aedes albopictus cells (C6/36) for the isolation of dengue virus in the in vitro transmission assay. Flavivirus viremias in suckling mice proved relatively noninfectious for Aedes aegypti. LLC-MK2 cells were adapted to a medium containing calf serum in order to conserve fetal calf serum. Cells were maintained and grown in calf serum; fetal calf serum was used only in the overlay medium used in the plague assay test.
Descriptors : *Dengue , *Vaccines , Culicidae , Disease vectors , Pathogenesis , In vitro analysis , Inoculation , Aedes , Infectious disease transmission , Immunoassay , Strains(Biology)
Subject Categories : Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE