Accession Number : ADA108884


Title :   A Role for Cytoplasmic Structural Proteins in the Transport of Water and Salts in the Intestine


Descriptive Note : Annual rept. 1 Feb 81-8 Dec 82


Corporate Author : BAYLOR COLL OF MEDICINE HOUSTON TX DEPT OF PHYSIOLOGY


Personal Author(s) : Beall, Paula T


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a108884.pdf


Report Date : 08 Dec 1981


Pagination or Media Count : 13


Abstract : This contract supports research to test the hypothesis - that the movement of water and salts across the epithelial membrane of the intestine depends not only on the properties of the cellular membranes but also on the structural properties of the cytoplasm of the cells. Current theories ascribe all controls of cellular transport of water and salts to passive diffusion and specialized enzymes, pores, and channels at the cell membrane. Classical interpretation allows no role for the cytoplasmic components of the cells in regulation or control of transport. Scattered experimental evidence produced in the 1970's, however, suggests that the disruption of the microtubule and microfilament network of the cytoplasm can have an effect on the rate and direction of transport. This proposal is in the process of testing the idea that the cytoskeleton plays a role along with water to regulate transport. For this purpose the intestinal epithelium has been chosen as a membrane through which large amounts of sodium and water pass. This work is part of a coordinated effort among three labs to study this phenomenum in vivo in perfused rat intestine, in vitro on stripped intestinal mucosa in Ussing chambers, and in isolated intestinal enterocytes. Preliminary data produced by this team has already resulted in the acceptance of a role for cytoplasmic proteins in the electrical properties of the intestinal epithelium.


Descriptors :   *PROTEINS , *CYTOPLASM , *DRUGS , EPITHELIUM , MEMBRANES(BIOLOGY) , RATS , WATER , ELECTRICAL PROPERTIES , TRANSPORT PROPERTIES , TRANSPORT , SALTS , INTESTINES , SECRETION


Subject Categories : Biochemistry
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE