Accession Number : ADA090844


Title :   Plasmid Isolation in Legionella pneumophila and Legionella-like Organisms.


Descriptive Note : Interim rept.,


Corporate Author : ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD


Personal Author(s) : Mikesell,Perry ; Ezzell,J W ; Knudson,Gregory B


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a090844.pdf


Report Date : 22 Aug 1980


Pagination or Media Count : 19


Abstract : Agarose gel electrophoresis was employed to screen nine strains of Legionella-like bacteria and one strain of Legionella pneumophila for the presence of extrachromosomal deoxyribonucleic acid. Cryptic plasmids were found in three of the isolates examined. A purification protocol was devised which consolidates the central tenets of several existing isolation techniques into a single procedure. Plasmid molecular weights were determined to range from 46.6 to 59.8 megadaltons (Mdal). (Author)


Descriptors :   *BACTERIA , *BACTERIAL DISEASES , ISOLATION , STRAINS(BIOLOGY) , GENETICS , ELECTROPHORESIS , ANTIBIOTICS


Subject Categories : Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE