Accession Number : ADA086136


Title :   Activation of Membrane-Bound Kallikrein and Renin in the Kidney.


Descriptive Note : Technical rept. 15 May 79-15 Aug 80,


Corporate Author : TEXAS UNIV HEALTH SCIENCE CENTER AT DALLAS


Personal Author(s) : Nishimura,Kazutaka ; Alhenc-Gelas,F ; White,A ; Erdos,E G


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a086136.pdf


Report Date : 23 May 1980


Pagination or Media Count : 26


Abstract : Rat kidney contains membrane-bound renin and kallikrein. Kallikrein activity was measured with a spectrophotometric assay and renin by radioimmunoassay. Plasma membrane-bound kallikrein was activated by lysolecithin and also by melittin, which was a more potent activator. This activation by mellitin was independent of calcium concentration. Melittin was, however, a more potent activator of bound-renin in the presence of calcium. Administration of aldosterone to rats for six days increased kallikrein activity in the renal homogenate and in the membrane-enriched fractions, while renin activity was not affected. It was proposed that kallikrein may be located also on the basal membrane of tubular epithelial cells, where aldosterone can enhance its activity. (Author)


Descriptors :   *RENIN , ACTIVATION , MEMBRANES(BIOLOGY) , RATS , CHEMICAL REACTIONS , KIDNEYS , ALDOSTERONE


Subject Categories : Biochemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE