Accession Number : AD1050830

Title :   Systems Biology of Immune Response to Live and Inactivated Dengue Virus Vaccines

Descriptive Note : Technical Report,01 Sep 2016,31 Aug 2017

Corporate Author : The Geneva Foundation Tacoma United States

Personal Author(s) : Currier, Jeffrey

Full Text :

Report Date : 01 Sep 2017

Pagination or Media Count : 25

Abstract : The objective of this project is to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive (T and B cell) responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach. During the first project year, IRB approvals, inter-institutional agreements, and measurements of dengue virus-specific neutralizing antibody titers and frequencies of cytokine-producing CD4 and CD8 T cells were completed. Flow cytometry of cellular activation and measurement of serum cytokine levels were completed on a subset of subjects. Pilot studies were done for RNA extraction and analysis of B cells and T cell repertoires.

Descriptors :   Systems Biology , vaccines , dengue , adaptive immunity , autoimmunity , lymphocytes , biological markers , epitopes

Subject Categories : Biology
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE