Accession Number : AD1050422


Title :   Systems Biology of the Immune Response to Live and Inactivated Dengue Virus Vaccines


Descriptive Note : Technical Report,01 Sep 2016,31 Aug 2017


Corporate Author : University of Rhode Island Kingston United States


Personal Author(s) : Currier, Jeffrey ; Rothman,Alan L


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1050422.pdf


Report Date : 01 Sep 2017


Pagination or Media Count : 25


Abstract : The objective of this project is to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive (T and B cell) responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach. During the first project year, IRB approvals, inter-institutional agreements, and measurements of dengue virus-specific neutralizing antibody titers and frequencies of cytokine-producing CD4 and CD8 T cells were completed. Flow cytometry of cellular activation and measurement of serum cytokine levels were completed on a subset of subjects. Pilot studies were done for RNA extraction and analysis of B cells and T cell repertoires.


Descriptors :   systems biology , vaccines , allergy and immunology , dengue , lymphocytes , adaptive immunity , antibodies , vs , blood chemistry , ribonucleic acids , biological markers , epitopes , clinical trials


Subject Categories : Medicine and Medical Research
      Biology
      Microbiology
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE