Accession Number : AD1050200

Title :   Single-Cell Dissection of Human Pancreatic Islet Dysfunction in Diabetes

Descriptive Note : Technical Report,01 Jun 2016,31 May 2017

Corporate Author : The Jackson Laboratory for Genomic Medicine Bar Harbor United States

Personal Author(s) : Stitzel, Michael L

Full Text :

Report Date : 01 Jun 2017

Pagination or Media Count : 55

Abstract : Islets are composed of 5 endocrine cell types that perform complementary functions to maintain proper glucose homeostasis. This cellular heterogeneity impedes our ability to understand the precise transcriptional repertoire and regulatory landscape of each cell type and to determine how these programs in each cell type are perturbed in type 2 diabetes (T2D). The overarching goal of this project is to determine, with single cell resolution, changes in cellular composition and cell-specific gene expression programs elicited by T2D in human islets using innovative single cell transcriptomic (scRNA-seq; Aim 1) and epigenomic (scATAC-seq; Aim 2) technologies.

Descriptors :   diabetes , epigenetics , sequence analysis , peptides , endocrine cells , glucose , homeostasis , gene expression

Subject Categories : Medicine and Medical Research
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE