Accession Number : AD1050200


Title :   Single-Cell Dissection of Human Pancreatic Islet Dysfunction in Diabetes


Descriptive Note : Technical Report,01 Jun 2016,31 May 2017


Corporate Author : The Jackson Laboratory for Genomic Medicine Bar Harbor United States


Personal Author(s) : Stitzel, Michael L


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1050200.pdf


Report Date : 01 Jun 2017


Pagination or Media Count : 55


Abstract : Islets are composed of 5 endocrine cell types that perform complementary functions to maintain proper glucose homeostasis. This cellular heterogeneity impedes our ability to understand the precise transcriptional repertoire and regulatory landscape of each cell type and to determine how these programs in each cell type are perturbed in type 2 diabetes (T2D). The overarching goal of this project is to determine, with single cell resolution, changes in cellular composition and cell-specific gene expression programs elicited by T2D in human islets using innovative single cell transcriptomic (scRNA-seq; Aim 1) and epigenomic (scATAC-seq; Aim 2) technologies.


Descriptors :   diabetes , epigenetics , sequence analysis , peptides , endocrine cells , glucose , homeostasis , gene expression


Subject Categories : Medicine and Medical Research
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE