Accession Number : AD1050124


Title :   Using High-Precision Signaling Activity Imaging to Personalize Ras Pathway Inhibition Strategies in Neurofibromatosis


Descriptive Note : Technical Report,01 Jun 2016,31 May 2017


Corporate Author : UNIVERSITY OF CALIFORNIA, DAVIS DAVIS United States


Personal Author(s) : Albeck, John ; Gillies,Taryn ; Blizard,Heather ; Pargett,Michael


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1050124.pdf


Report Date : 01 Jun 2017


Pagination or Media Count : 12


Abstract : The goal of this project is to develop reporter cells and to perform real-time ERK and Akt activity measurements in cell types and genetic contexts relevant to NF1. During this award period, we have improved methodologies for introducing genetically encoded signaling reporters into cells and for performing quantitative analysis of signals in individual living cells. We have developed an astrocyte cell line expressing ERK and Akt reporters and have characterized the response of this cell line to growth factor stimulation and to pharmacological inhibitors of Ras pathway effectors, revealing a surprisingly high level of basal (unstimulated) ERK activity in these astrocytic cells relative to epithelial cells and fibroblasts. The methods and initial data collected in this period will allow us to perform a detailed characterization of Ras effector signaling in normal and NF1-mutant cells over the next year of the project.


Descriptors :   genetic disorders , neoplasms , inhibitors , kinetics , mutations , inhibition , therapeutics


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE