Accession Number : AD1048375


Title :   Lung Injury; Relates to Real-Time Endoscopic Monitoring of Single Cells Respiratory Health in Lung


Descriptive Note : Technical Report,01 Sep 2016,31 Aug 2017


Corporate Author : Childrens Hospital Los Angeles Los Angeles United States


Personal Author(s) : Warburton,David ; Moats,Rex ; Turcatel,Gianluca ; Fraser,Scott ; Cutrale,Francesco


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1048375.pdf


Report Date : 01 Sep 2017


Pagination or Media Count : 24


Abstract : The goal of this proposal is to design a portable bronchoscope that can determine the health status of the tracheal epithelial lining cells by analyzing changes in their metabolic profile. The device is intended to be used to quickly single out which individuals is showing injury and inflammation in the trachea and lung and therefore to provide them with the most adequate and early care. In this closing year 1 funding period, we have built the bronchoscope and done some preliminary testing on mouse trachea samples exposed to chemicals known to injure the tracheal epithelium. The size of the bronchoscope is quite small and will allow its transport and use by the military in the field or remote locations, while the small size of the instrument could permit its use on awake patients. Following evaluation of the field of research we have decided to develop a hybrid system Camera and Raster with two modalities: seek and focus. The former modality will allow low magnification scan of the trachea and lung airway to search for areas of interest that will then be analyzed in greater detail using the latter modality. Our preliminary data using tissue reflectance on mouse tracheal tissue have shown that our instrument can, indeed, successfully separate injured tracheal epithelial cells from the healthy ones without the use of additional reagents or chemicals.


Descriptors :   lung , wounds and injuries , endoscopy , HYPERSPECTRAL IMAGERY , fluorescence , naphthalenes , TRACHEA , EPITHELIAL CELLS , inflammation , EPITHELIUM , calibration


Subject Categories : Medicine and Medical Research
      Anatomy and Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE