Accession Number : AD1048149


Title :   Mouse and Human Models for Investigating Influences of Tau on Progression of Alzheimer's Disease Following Traumatic Neuronal Injury


Descriptive Note : Technical Report,15 Sep 2016,14 Sep 2017


Corporate Author : University of California, San Diego La Jolla United States


Personal Author(s) : Shah, Sameer B


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1048149.pdf


Report Date : 01 Oct 2017


Pagination or Media Count : 10


Abstract : We have completed the design, fabrication, and validation of a new biomedical device to impose moderate mechanical loads on cultured stem-cell derived neuronal and glial cells. Using this device, we assessed morphological changes, beta amyloid production, and tauphosphorylation (i.e., multiple Alzheimer's-associated outcomes) following rapid stretch of iPSC-derived neurons. Results suggest that neurites oriented in the direction of substrate stretch were non-elastically deformed, the cytoskeleton was reorganized, a myloid precursor protein transport was perturbed, amyloid production was increased, and tau phosphorylation unchanged following a single bout of mechanical loading. Multiple bouts of mechanical loading amplified amyloid and tau phenotypes, suggesting a dependence of these Alzheimer's associated outcomes to injury dose or severity.


Descriptors :   brain injuries , biomechanics , neurons , ALZHEIMER DISEASE , stem cells , AMYLOID , MICROFLUIDICS , CYTOKINES , inflammation


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE