Accession Number : AD1044275


Title :   Identifying Neurofibromin-Specific Regulatory Nodes for Therapeutic Targeting in NF1


Descriptive Note : Technical Report,30 Sep 2015,29 Sep 2016


Corporate Author : University of California San Francisco San Francisco United States


Personal Author(s) : McCormick,Frank P ; Markegard,Evan ; Tetsu,Osamu ; Mercado,Ellen


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1044275.pdf


Report Date : 01 Oct 2016


Pagination or Media Count : 21


Abstract : The overall goal of the project is to determine how neurofibromin is regulated, and to explore the hypothesis that loss of neurofibromin activity leads to up-regulation of specific receptors. We are building on our earlier discovery, that neurofibromin depends on the adapter protein SPRED1, to function, and we are utilizing the latest technical innovations including CRISPR technology to find genes that regulate neurofibromin SPRED function. To date we have demonstrated that oncogenic EGFR signaling disrupts Spred1-NF1 binding. Mass spectrometry was performed on cells overexpressing EGFRL858R to identify potential phosphorylation sites on Spred1 and NF1 that could disrupt Spred1-NF1 binding by steric hindrance.


Descriptors :   cancer research , therapeutics , targets , genetics , inhibition


Subject Categories : Genetic Engineering and Molecular Biology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE