Accession Number : AD1042147


Title :   Coupling Spatial Segregation with Synthetic Circuits to Control Bacterial Survival (Open Access)


Descriptive Note : Journal Article


Corporate Author : DUKE UNIV DURHAM NC DURHAM United States


Personal Author(s) : Huang,Shuqiang ; Lee,Anna J ; Tsoi,Ryan ; Wu,Feilun ; Zhang,Ying ; Leong,Kam W ; You,Lingchong


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1042147.pdf


Report Date : 29 Feb 2016


Pagination or Media Count : 13


Abstract : Engineered bacteria have great potential for medical and environmental applications. Fulfilling this potential requires controllability over engineered behaviors and scalability of the engineered systems. Here, we present a platform technology, microbial swarmbot, which employs spatial arrangement to control the growth dynamics of engineered bacteria. As a proof of principle, we demonstrated a safeguard strategy to prevent unintended bacterial proliferation. In particular, we adopted several synthetic gene circuits to program collective survival in Escherichia coli: the engineered bacteria could only survive when present at sufficiently high population densities. When encapsulated by permeable membranes, these bacteria can sense the local environment and respond accordingly. The cells inside the microbial swarmbot capsules will survive due to their high densities. Those escaping from a capsule, however, will be killed due to a decrease in their densities. We demonstrate that this design concept is modular and readily generalizable. Our work lays the foundation for engineering integrated and programmable control of hybrid biologicalmaterial systems for diverse applications.


Descriptors :   bacteria , biotechnology , synthetic biology , bioengineering , cells(biology) , cellular structures , systems biology , dynamics


Subject Categories : Biomedical Instrumentation and Bioengineering


Distribution Statement : APPROVED FOR PUBLIC RELEASE