Accession Number : AD1041932


Title :   Testing of Ruggedized Antibodies within a Lateral Flow Immunoassay


Descriptive Note : Technical Report,01 Feb 2015,01 Sep 2015


Corporate Author : Director, ECBC APG United States


Personal Author(s) : Calm,Alena M ; Thompson,Roy ; Betts,Kelley ; Hofmann,Randy ; Chen,Hsiu ; Ford,Glen ; Ma,Joe


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1041932.pdf


Report Date : 01 Oct 2017


Pagination or Media Count : 24


Abstract : Antibody stability is a key quality parameter for a number of DoD customers who rely on effective and fielded hand-held immunoassays (HHAs). This project leverages antibody ruggedization efforts at U.S. Army Edgewood Chemical BiologicalCenter (ECBC) to determine whether the ruggedization of detection antibodies translates into improved and more fieldable assays. Capitalizing on the work performed at ECBC in collaboration with the Defense Advanced Research Projects Agency,Antibody Technology Program and the Defense Threat Reduction Agency, Ruggedized Antibody Program, this effort was projected to develop a lateral flow immunoassay against the ricin toxin and increase thermostability that enlists the use of asmartCAR reader device, which is capable of communicating results with a smartphone. Incorporating previously ruggedized ricin antibodies into fieldable HHAs potentially makes these assays more amenable to forward-deployed hazard detection and eliminates (or greatly reduces) the current requirement for 4 C cold-chain logistics.


Descriptors :   antibodies , immunoassay , ricin , biotechnology , biological detection , medical research , biological warfare agents , biosensors , immunoassay


Subject Categories : Medicine and Medical Research
      Chemical, Biological and Radiological Warfare


Distribution Statement : APPROVED FOR PUBLIC RELEASE