Accession Number : AD1041669


Title :   Engineering Probiotics that Improve Warfighter Performance by Maintaining Lean Body Mass and Inhibiting Anxiety


Descriptive Note : Technical Report,15 May 2014,14 May 2017


Corporate Author : William Marsh Rice University Houston United States


Personal Author(s) : Tabor,Jeffrey J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1041669.pdf


Report Date : 03 Oct 2017


Pagination or Media Count : 10


Abstract : The overall goal of this work is to engineer synthetic probiotics: orally-administered gut bacteria that sense and compute the metabolic signature of a specific disease in the colon, and respond by secreting a therapeutic compound that treats the molecular basis of the disease. Here, we combined computation and experiments to discover the first known biological sensor of thiosulfate, a two-component system from S. halifaxensis. We transferred this sensor into the gut-adapted strain E. coli Nissle 1917 and used it to control GFP expression. We then administered this sensor strain to healthy mice and mice with colitis, and demonstrated that our sensor bacteria reliably report colitis. This work is a major advance toward our vision.


Descriptors :   synthetic biology , biosensors , cell physiological processes , inflammation , hidden markov models , computational biology , electron acceptors , systems biology , digestive system processes , Anxiety , body weight , bacteria , military research , colitis


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE