Accession Number : AD1039316


Title :   SIGNALING PATHWAYS ASSOCIATED WITH VX EXPOSURE IN MESENCHYMAL STEM CELLS


Descriptive Note : Technical Report,01 Mar 2016,31 Oct 2016


Corporate Author : EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND United States


Personal Author(s) : Angelini,Daniel ; Phillips,Christopher ; Prugh,Amber ; Glaros,Trevor ; Tran,Tran


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1039316.pdf


Report Date : 01 Sep 2017


Pagination or Media Count : 34


Abstract : Mesenchymal stem cells (MSCs) are multipotent adult stem cells that are key regulators of tissue maintenance and repair. These cells have been identified in several different tissues, but they typically originate in the bone marrow and then circulate through the body in the bloodstream. The U.S. Army Edgewood Chemical Biological Center BioDefense Branch members and academic laboratories have demonstrated that bone marrow-derived MSCs exposed to organophosphate (OP) pesticides sustain significant changes in their ability to proliferate and differentiate. In the literature, OP compounds were shown to affect the activity and expression levels of acetylcholinesterase (AChE); however, it is still unclear whether the effect on AChE is caused by these changes. It is also possible that these OP compounds have other unknown secondary effects on MSCs. In this study, we exposed MSCs to the OP chemical warfare agent 145O-ethyl S-(2-diisopropylaminoethyl) methylphosphonothioate (VX) and performed phospho-array and phosphoproteomic analysis to gain insights into the signaling pathways associated with VX exposure.


Descriptors :   stem cells , chemical warfare agents , Toxicity , Exposure (Physiology) , proteins , proteomics , liquid chromatography , Mass Spectrometry


Subject Categories : Anatomy and Physiology
      Chemical, Biological and Radiological Warfare


Distribution Statement : APPROVED FOR PUBLIC RELEASE