Accession Number : AD1036300


Title :   Next Generation Molecular Histology Using Highly Multiplexed Ion Beam Imaging (MIBI) of Breast Cancer Tissue Specimens for Enhanced Clinical Guidance


Descriptive Note : Technical Report,01 Jul 2015,30 Jun 2016


Corporate Author : University of California, Davis Davis United States


Personal Author(s) : Borowsky,Alexander


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1036300.pdf


Report Date : 01 Jul 2016


Pagination or Media Count : 17


Abstract : We have completed the goals of the project timeline, in large part, in this second period of the grant funding. Specifically, we have developed the reagents, cell lines and tissues needed for the assessment of the dynamic range of protein quantitation. We have analyzed the lines with roughly 2/3rds of the planned assays, with the remaining l/3rd in progress. We have optimized multiplexed IF for comparison to MIBI secondary ion mass spectrometry. And we have evaluated mRNA ISH probes for use in both IF and MIBI detection. The Stanford group in related but not funded by this proposal work has developed a second generation SIMS instrument which will enable higher throughput, and facilitate the further aims of the proposal in the coming grant periods.


Descriptors :   breast cancer , histology , Pathology , Morphology , ion beams


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE