Accession Number : AD1035684


Title :   Optimization of Lyophilized Plasma for Use in Combat Casualties


Descriptive Note : Technical Report,29 Dec 2010,28 Dec 2015


Corporate Author : Oregon Health and Science University Portland United States


Personal Author(s) : Schreiber,Martin A ; Underwood,Samantha


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1035684.pdf


Report Date : 01 Mar 2016


Pagination or Media Count : 26


Abstract : Purpose: Lyophilized plasma (LP) is a light weight powder that remains stable for a prolonged period of time and in a broad range of temperatures. The powder is rapidly reconstituted into a product that preserves coagulation factor activity and suppresses harmful inflammation. The purpose of this project was to demonstrate that LP could be optimized by minimizing the reconstitution fluid and improving the constituents. Scope: Specific aim 1 focused on determining the minimum amount of fluid required to reconstitute a safe and effective product. Specific aim 2 evaluate four fluid options for reconstitution. Specific aim 3 evaluated the concentration of ascorbic acid used to buffer the LP and potentially reduce the amount of inflammation due to injury. Finally, specific aim 4 involved development of a survival model that utilized the final optimized LP product. Findings: LP can be safely reconstituted with 50% of the original volume with sterile water being the best fluid for reconstitution. Ascorbic acid can safely be used to buffer the LP, and the concentration does not make a difference.


Descriptors :   models , hemorrhage , BLOOD PLASMA , BLOOD COAGULATION , pigs , inflammation , deoxyribonucleic acids , FLUIDS


Subject Categories : Biochemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE