Accession Number : AD1034907


Title :   Effect of 2 Bedding Materials on Ammonia Levels in Individually Ventilated Cages


Descriptive Note : Journal Article - Open Access


Corporate Author : U.S. Anny Center for Environmental Health Research Fort Detrick United States


Personal Author(s) : Koontz,Jason M ; Kumsher,David M ; Kelly,Richard III ; Stallings,Jonathan D


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1034907.pdf


Report Date : 01 Jan 2016


Pagination or Media Count : 5


Abstract : This study sought to identify an optimal rodent bedding and cage-change interval to establish standard procedures for the IVC in our rodent vivarium. Disposable cages were prefilled with either corncob or -cellulose bedding and were used to house 2 adult SpragueDawley rats (experimental condition) or contained no animals (control). Rats were observed and intracage ammonia levels measured daily for 21 d. Intracage ammonia accumulation became significant by day 8 in experimental cages containing -cellulose bedding, whereas experimental cages containing corncob bedding did not reach detectable levels of ammonia until day 14. In all 3 experimental cages containing -cellulose, ammonia exceeded 100 ppm (our maximum acceptable limit) by day 11. Two experimental corncob cages required changing at days 16 and 17, whereas the remaining cage containing corncob bedding lasted the entire 21 d without reaching the 100-ppm ammonia threshold. These data suggests that corncob bedding provides nearly twice the service life of -cellulose bedding in the IVC system.


Descriptors :   laboratory animals , measurement , CELLULOSE , detection , environmental health , bacteria , biomedical research


Subject Categories : Inorganic Chemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE