Accession Number : AD1033923


Title :   Methods Development for the Isolation and Culture of Primary Corneal Endothelial Cells


Descriptive Note : Technical Report,01 Jan 2011,30 Sep 2016


Corporate Author : U.S. Army Medical Research Institute of Chemical Defense Aberdeen Proving Ground United States


Personal Author(s) : Haggins,Robert Jr L ; Eaton,Erik Jr B ; Galvez,Victor J ; Varney,Timothy R


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1033923.pdf


Report Date : 01 Feb 2017


Pagination or Media Count : 15


Abstract : The vesicating agent sulfur mustard (SM) continues to represent a worldwide threat because of the compound's ease of production, minimal cost of manufacturing, widespread stockpiling and the lack of definitive medical treatment. The vast majority of SM exposure cases involve corneal injury. At low-exposure doses, ocular irritation and inflammation will resolve within weeks and generally will not recur. However, higher exposure levels may result in a second phase of pathology that can arise decades after initial symptoms have resolved. These patients experience repeated cycles of severe edema, corneal erosion, pathological angiogenesis and corneal scarring that can eventually result in blindness. The medical complications of late-onset ocular SM injury are collectively referred to as mustard gas keratopathy (MGK). Prevailing evidence suggests that late onset MGK may result from a deficit in corneal endothelial cells (CECs). If so, cellular therapy intended to replenish CECs lost to injury and age may represent an effective approach in the treatment or prevention of MGK onset. Here we discuss a systematic evaluation of in vitro CEC propagation methods. These investigations define optimized protocols for CEC culture expansion to pharmaceutically relevant cell numbers, enabling cell replacement therapy as a potential option in the treatment of MGK.


Descriptors :   eye , cornea , epithelial cells , epithelium , tissues , therapeutics , chemical warfare agents , growth factors , in vitro analysis , exposure(general) , wounds and injuries , blindess


Subject Categories : Weapons Effects(biological)
      Anatomy and Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE