Accession Number : AD1033889


Title :   Potential Therapeutic Use of Relaxin in Healing Cranial Bone Defects


Descriptive Note : Technical Report,20 Jul 2015,19 Jul 2016


Corporate Author : University of Florida Gainesville United States


Personal Author(s) : Conrad,Kirk P


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1033889.pdf


Report Date : 01 Aug 2016


Pagination or Media Count : 10


Abstract : The overall objective is to provide proof-of-principle that recombinant human relaxin (rhRLX) administration will accelerate bone healing in a calvarial defect model in mice by promoting angiogenesis/vasculogenesis and osteogenesis, at least in part through incorporation of bone marrow-derived angio- and osteogenic progenitor cells into the lesion. Results from the initial study conducted during this reporting period demonstrated: successful production of chimeric mice after irradiation and GFP+ bone marrow transplantation; reproducible implementation of uniform cranial lesions of 1.5 mm diameter, and circulating concentrations of relaxin ranging from 30-80 ng/ml. However, after 11 weeks of healing, the lesion closure was comparable in the relaxin- and vehicle-treated mice (70%). Therefore, in the next study we will decrease the healing time to 4 and 8 weeks in order to assess whether the closure occurs more rapidly with relaxin treatment. In view of the biphasic dose response curve for relaxin, which is dependent on the functional endpoint, we will also reduce the infusion rate by 20-fold to 0.05 microgram/h, which in our experience should produce a circulating relaxin concentration of 1-5 ng/ml.


Descriptors :   bone marrow , medical personnel , stem cells , xray computed tomography , bone marrow cells , surgical procedures , growth factors , bone fractures , culture techniques , osteogenesis , biomedical research , blood vessels , bone and bones , therapeutics


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE