Accession Number : AD1033234


Title :   Systematic Identification of Genes Required for Expression of Androgen Receptor Splice Variants


Descriptive Note : Technical Report,01 Jun 2015,31 May 2016


Corporate Author : University of Mississippi Medical Center Jackson United States


Personal Author(s) : Mo,Yin-Yuan


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1033234.pdf


Report Date : 01 Aug 2016


Pagination or Media Count : 23


Abstract : Recently, a new genetic engineering tool has been developed from bacterial Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated System (Cas). It has several advantages over the existing ones and one of them is the ability to make a very large number (tenth of thousands) of gene knockouts so that we can screen genes important to prostate cancer. For example, androgen receptor (AR) is well known for its role in prostate cancer progression and castration resistance, and several AR splicing variants have been identified in castration resistant prostate cancer. With this technology, we can answer questions like how prostate cancer becomes castration resistant after anti-hormone therapy. Furthermore, these genes screened out with this technology may serve biomarkers or therapeutic targets. Thus, the overall of goal of this application is to identify genes involved in regulation of AR splice variant through CRISPR/Cas screening system.


Descriptors :   prostate cancer , neoplasms , androgens , biological markers , genes


Subject Categories : Genetic Engineering and Molecular Biology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE