Accession Number : AD1031105


Title :   Acceleration of Regeneration of Large Gap Peripheral Nerve Injuries Using Acellular Nerve Allografts plus amniotic Fluid Derived Stem Cells (AFS)


Descriptive Note : Technical Report,01 Sep 2015,31 Aug 2016


Corporate Author : Wake Forest University Health Sciences Winston-Salem United States


Personal Author(s) : Li,Zhongyu ; Smith,Thomas L


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1031105.pdf


Report Date : 01 Sep 2016


Pagination or Media Count : 42


Abstract : Major accomplishments this year include successful seeding of AFS into ANA. This accomplishment also documented that these cells remained viable up to 72 hours after seeding. The seeded constructs were used to repair critical-sized, large gap nerve injury in rats and their functional recovery was monitored longitudinally using digital video gait analysis as well as electrophysiologic and histologic outcomes. The data analysis is not complete, but preliminary data indicate that this AFS seeded ANA used for nerve repair results in an improved functional outcome for the rats compared to a nerve autograft, the current gold standard for tension-free repair of transected peripheral nerves. The coming year will utilize these techniques for repairing large-gap (6 cm) nerve injuries in non-human primates. These represent a more translational model of peripheral nerve injury and repair.


Descriptors :   peripheral nervous system , transplants , stem cells , rats


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE