Accession Number : AD1030317


Title :   Development of Purification Protocol Specific for Bacteriocin 105B


Descriptive Note : Technical Report,01 Nov 2013,31 Aug 2014


Corporate Author : ARMY NATICK SOLDIER RESEARCH DEVELOPMENT AND ENGINEERING CENTER MA NATICK United States


Personal Author(s) : Stote,Robert ; Rego,Jennifer M ; Cowell,Courtney M


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1030317.pdf


Report Date : 09 Feb 2017


Pagination or Media Count : 20


Abstract : This report summarizes work conducted to establish a purification procedure specific to a narrow-spectrum antimicrobial peptide. This antimicrobial agent, termed a bacteriocin, was evaluated in previous work and determined to exhibit activity against a pathogenic organism of interest to the Army, Bacillus anthracis Sterne, a surrogate of the active form of Bacillus anthracis. As the current application of broad-spectrum antimicrobials promotes the development of multi-drug resistant microorganisms, targeted antimicrobials are being investigated as a suitable alternative. Although bacteriocins have exhibited great promise as narrow-spectrum antimicrobial agents, their wide-spread adaptation has been limited in part due to the time-consuming and costly processes employed for development. The work cited in this report presents a robust purification procedure for an individual bacteriocin to maximize the yield of the antimicrobial peptide for employment in future assays and development into a platform, such as a textile, that relays antimicrobial activity.


Descriptors :   antiinfective agents , test and evaluation , peptides , biological sciences , bacteria , cells(biology) , cultures(biology)


Distribution Statement : APPROVED FOR PUBLIC RELEASE