Accession Number : AD1030123

Title :   Amplifying Riboswitch Signal Output using Cellular Wiring

Descriptive Note : Technical Report,01 Feb 2014,30 Jun 2016

Corporate Author : UES, Inc. Dayton United States

Personal Author(s) : Goodson,Michael S ; Bennett,Annastacia C ; Jennewine,Brenton R ; Briskin,Emily ; Harbaugh,Svetlana V ; Kelley-Loughnane,Nancy

Full Text :

Report Date : 30 Jan 2017

Pagination or Media Count : 15

Abstract : If fieldable riboswitch-based biological sensors are to fulfill their potential, it is necessary to increase their signal output. Here we report a novel modular amplification system using a riboswitch to initiate signaling between a sensing strain and a reporter strain of E. coli. A quorum sensing signaling molecule biologically wires the sensing and reporter strains together. The amplification circuit increased the amount of fluorescence generated on ligand binding compared to when the riboswitch controlled fluorescence expression directly.

Descriptors :   Amplification , Biosensors , SIGNAL PROCESSING , GENETIC ENGINEERING , ligandS

Distribution Statement : APPROVED FOR PUBLIC RELEASE