Accession Number : AD1025978


Title :   Epigenetic Mediation of Endocrine and Immune Response in an Animal Model of Gulf War Illness


Descriptive Note : Technical Report,30 Sep 2015,29 Sep 2016


Corporate Author : University of Toronto Toronto Canada


Personal Author(s) : McGowan,Patrick O ; Broderick,Gordon ; O'Callaghan,James ; Morris,Mariana ; Fletcher,Mary A


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1025978.pdf


Report Date : 01 Oct 2016


Pagination or Media Count : 19


Abstract : There are now compelling human epidemiological and animal experimental data that indicate the risk of developing complex diseases is influenced by persistent epigenetic adaptations in response to environmental exposures such as toxins and stress. We propose to examine the epigenomic response to diisopropyl fluorophosphates (DFP), a sarinsurrogate, and associated changes to the immune and endocrine response to lipopolysaccharide (LPS) challenge in amouse model of Gulf War Illness (GWI), with stress hormone exposure as an experimental mediator. We will study the relationship between changes in DNA methylation and chromatin modifications in peripheral blood and the brain (specifically hippocampus and prefrontal cortex) in order to pursue a mechanistic understanding of the underlying pathology of GWI. During this reporting period, we have begun data collection on DNA methylation modifications and gene expression profiles in the brains of mice exposed to saline control, corticosterone and DFP, and have developed and refined protocols for this project in line with the GWIRC sister project.


Descriptors :   gene expression , genomics , therapeutics , environmental exposure , stem cells , dna sequence analysis , immune system , methylation , cell lineage , chromosome structures , biological processes , brain , neuroglia , genetic structures , experimental data , STRESS (PHYSIOLOGY) , toxins , immune system


Subject Categories : Medicine and Medical Research
      Stress Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE