Accession Number : AD1025908

Title :   STEM VQ Method, Using Scanning Transmission Electron Microscopy (STEM) for Accurate Virus Quantification

Descriptive Note : Journal Article

Corporate Author : USAMRIID Frederick United States

Personal Author(s) : Blancett,Candace D ; Fetterer,David P ; Koistinen,Keith A ; Morazzani,Elaine M ; Monninger,Mitchell K ; Piper,Ashley E ; Kuehl,Kathy A ; Kearney,Brian J ; Norris,Sarah L ; Rossi,Cynthia A ; Glass,Pamela J ; Sun,Mei G

Full Text :

Report Date : 02 Feb 2017

Pagination or Media Count : 21

Abstract : Accurate virus quantification is sought, but a perfect method still eludes the scientific community. Electron Microscopy (EM) quantification is a valuable technique because it provides morphology data and counts all viral particles, including partial or noninfectious particles; however, EM methods have been criticized as inaccurate, non-reproducible, and having detection limits too high to be useful. To address these issues, we developed a consistent, reproducible virus quantification method called Scanning Transmission Electron Microscopy Virus Quantification (STEM-VQ) which simplifies sample preparation and utilizes large throughput STEM imaging and commercially available software to count particles. In this paper, we present data evaluating alphavirus viral stock preparations that supports this methods reproducibility and accuracy including a comparison of this quantification method to viral plaque assay and the ViroCyt Virus Counter.

Descriptors :   transmission electron microscopy , essential amino acids , cell line , accuracy , electron microscopy , computer programs , equine encephalitis , arbovirus infections , viruses

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE