Accession Number : AD1025426


Title :   Tailoring Gut Microbiota for Enhanced Resilience and Performance Under Sleep-Deprived Conditions


Descriptive Note : Technical Report,02 Jun 2014,30 Sep 2016


Corporate Author : AIR FORCE RESEARCH LAB WRIGHT PATTERSON AFB OH HUMAN PERFORMANCE WING (711TH) WRIGHT PATTERSON AFB United States


Personal Author(s) : Soto,Armando ; Chan,Victor T ; Walters,Amy D ; Dixon,David ; Braddock,Amber ; Hill,Emily E ; Shurte,Leah ; Sayson,Steven E ; Soto,Jonathon


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1025426.pdf


Report Date : 01 Aug 2016


Pagination or Media Count : 25


Abstract : Sleep deprivation has been associated with an increase in overall mortality and morbidity. Mechanistically, a loss of brain mass, due to neuronal death, has been observed after chronic sleep deprivation. Sleep deprivation also has enormous impacts on metabolic homeostasis, with increased prevalence of weight gain and obesity. Interestingly, perturbation of gut microbiota presents a pattern of metabolic abnormalities mirroring those induced by sleep deprivation. In this study, perturbation of gut microbiota and host functions following sleep deprivation will first be determined, using a humanized rat model, which is derived by transplanting native human gut microbiota into germ-free rats. This microbiota perturbation effect is based upon our hypothesis that sleep deprivation initially causes degradation in the functional and structural integrity of the gastrointestinal tract. Data generated will be used to develop a predictive metabolic network model of host-microbiota interaction. This model will be validated under various conditions of gut microbiota modulations using prebiotic, probiotic and synbiotic approaches. The validated model will be used to identify targets that can effectively tailor the gut microbiota and counter the adverse effects of sleep deprivation, resulting in enhanced host resilience and performance. This study is a four year project, the current report describes the accomplishments during the first year including establishment of the rodent germ-free facilities, selection of the human donors, and the creation of the pseudo germ-free animal model. This report also describes the work plan and future direction of the project.


Descriptors :   sleep deprivation , body weight , biological factors , brain , bacteria


Subject Categories : Stress Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE