Accession Number : AD1025345


Title :   Development of Biomarkers for Assessing In Situ RDX Biodegradation Potential


Descriptive Note : Technical Report,01 Feb 2012,31 Aug 2016


Corporate Author : Michigan State University East Lansing United States


Personal Author(s) : Cupples,Alison M


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1025345.pdf


Report Date : 10 Jun 2016


Pagination or Media Count : 108


Abstract : The objective was to identify the microorganisms and genes responsible for the biodegradation of RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) in mixed microbial communities using stable isotope probing, quantitative PCR and high throughput sequencing. The research targeted RDX biodegradation in mixed community samples, as these microbial communities are more representative of those at contaminated sites. The research involved four major projects, the first of which was a literature review. The second task examined the RDX degrading communities in four different soil slurries. The third task examined the microorganisms involved in RDX biodegradation from surface soils from a detonation area at a Navy Base. The fourth task investigated the microorganisms and functional genes (xenA, xenB and xplA) linked to RDX biodegradation at two Navy sites. Several key microorganisms were associated with RDX removal in these mixed communities. These phylogenetic and functional biomarkers will be useful to determine the biological potential for RDX degradation across sites.


Descriptors :   Microorganisms , Genes , biodegradation , Soils , Naval shore facilities , Stable isotopes , Bacillaceae , proteobacteria , Bacteria


Subject Categories : Soil Mechanics


Distribution Statement : APPROVED FOR PUBLIC RELEASE