Accession Number : AD1025166


Title :   UNC Pembroke Laser Scanning Confocal Microscopy Facility


Descriptive Note : Technical Report,01 Feb 2015,31 Jan 2016


Corporate Author : University of North Carolina at Pembroke Pembroke United States


Personal Author(s) : Bahr,Ben A


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1025166.pdf


Report Date : 29 Apr 2016


Pagination or Media Count : 15


Abstract : UNC-Pembroke received funds from the Department of Defense Research and Education Program for Historically Black Colleges and Universities and Minority-Serving Institutions (Equipment/Instrumentation) in order to acquire a Nikon C2+ Confocal Microscopy System. The system strengthens research at UNC-Pembroke, by enhancing the Universitys Sample Preparation and Microscopy Facility with the capability of laser scanning confocal microscopy. The resulting system has the imaging and resolution capabilities needed to effectively analyze early cellular and cytoskeletal damage, as well as associated synaptic deterioration. The microscope was updated with the Nikon Perfect Focus component to allow live cell/intracellular organelle imaging for long-term resolution of cellular changes in different disease states. The funds were also used for 1) transgenic mice housing systems to accommodate mouse strains for addressing experimental objectives in Army projects (RDX blasts, paraoxon exposure), 2) a state-of-the-art immunoblot scanning device in order to compare to histology images and to maximize data obtained from small groups of blast and toxin-treated samples, and 3) a guillotine and freezer for tissue preparation and storage. The Facility supports undergraduate students to engage in cutting edge research and acquire the skills and experience to prepare them for graduate programs and research careers in DOD-relevant areas.


Descriptors :   CONFOCAL MICROSCOPY , brain , confocal microscopy , brain injuries , wounds and injuries , explosives


Subject Categories : Optics
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE