Accession Number : AD1024842

Title :   Acquisition of a Circular Dichroism Spectrometer to Study Biological Molecules at Interfaces

Descriptive Note : Technical Report,15 Jul 2014,14 Jan 2016

Corporate Author : University of Michigan - Ann Arbor Ann Arbor United States

Personal Author(s) : Chen,Zhan

Full Text :

Report Date : 10 Feb 2016

Pagination or Media Count : 15

Abstract : We have made substantial progress in the design, implementation, and use of our purchased Circular Dichroism (CD) system and the humidity control device. Currently this CD spectrometer can be used to study peptide/proteins in solution, on surface, and at different humidity levels. We successfully set up a humidity control system which can control the humidity of the CD sample compartment. Humid air is harmful to the optical components of the CD spectrometer. Therefore instead of building a humidity control system inside the CD spectrometer, we built an air tight chamber in the sample compartment, a humid air balance box with humidity control device outside of the CD spectrometer. We connected these two parts using light resistant tubes. We successfully applied this newly installed CD system to study various surface immobilized peptides and proteins. We successfully studied secondary structures of surface immobilized peptides/proteins with different surface immobilization methods, on different immobilization substrates, at different environmental conditions, and with or without co-immobilized hydromimetic functionalities.

Descriptors :   Spectrometers , Biomolecules , immobilized proteins , laboratory equipment

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Test Facilities, Equipment and Methods
      Atomic and Molecular Physics and Spectroscopy

Distribution Statement : APPROVED FOR PUBLIC RELEASE