Accession Number : AD1024764


Title :   Targeting Histone Abnormality in Triple Negative Breast Cancer


Descriptive Note : Technical Report,01 Aug 2015,31 Jul 2016


Corporate Author : University of Pittsburgh Pittsburgh United States


Personal Author(s) : Huang,Yi


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1024764.pdf


Report Date : 01 Aug 2016


Pagination or Media Count : 33


Abstract : In this funding period, we continued to test the hypothesis that silencing of key tumor suppressive genes by enhanced crosstalk between LSD1 and HDAC5 is a unique epigenetic mechanism promoting TNBC growth, and blockade of the HDAC5-LSD1 axis results in profound inhibition of TNBC growth and metastasis. By using In vitro pull-down assays with His-tag recombinant LSD1 protein incubating withHDAC5 full-length or deletion mutants and immunofluorescence assays, we identified that HDAC5 domain containing nuclear localization element is essential for interaction with LSD1.


Descriptors :   breast cancer , Histones , therapeutics , neoplasms , gene expression


Subject Categories : Biochemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE