Accession Number : AD1024139


Title :   Characterization and Expression of Drug Resistance Genes in MDROs Originating from Combat Wound Infections


Descriptive Note : Technical Report,13 Sep 2012,12 Jun 2016


Corporate Author : J Craig Venter Institute Rockville United States


Personal Author(s) : Fouts,Derrick E ; Chan,Agnes


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1024139.pdf


Report Date : 01 Sep 2016


Pagination or Media Count : 57


Abstract : Twenty-five multi-drug resistant organisms (MDROs) of high military importance were sequenced using the Illumina HiSeq platform to identify drug resistance and virulence mechanism contributing to their success as pathogens in the wound environment. A novel bioinformatics pipeline was used to incorporate genomic sequencing data obtained from multiple sequencing platforms with optical restriction map data to assemble the genomes into sequence contigs. Bioinformatics processes for the automated identification and classification of antibiotic resistance mechanisms and IS elements were developed to enable mapping of these features onto the genomes of the 25 selected MDROs. Preliminary investigations of IS element content showed considerable diversity and abundance levels across a collection of Acinetobacter genomes. Three top priority MDROs and three commensal microbes were chosen and obtained from WRAIR MRSN for in vitro co-culturing in confrontation assays aimed at investigating alterations in gene expression, particularly antibiotic resistance genes and virulence factors. Mock confrontations using E. coli and S. aureus controls have been conducted for the development of a new solid-media based confluent lawn co-culturing assay, and an RNA extraction protocol that is biologically reproducible. Human cell cultures to be used in bacterial-human cell confrontations have been received and grown successfully in the lab.


Descriptors :   DRUG RESISTANCE , wounds and injuries , combat injuries , military medicine , BIOMEDICAL INFORMATION SYSTEMS , Antibiotics , wound infections , gene expression , sequence analysis


Subject Categories : Medicine and Medical Research
      Stress Physiology
      Pharmacology


Distribution Statement : APPROVED FOR PUBLIC RELEASE