Accession Number : AD1022557


Title :   In Situ Bioremediation of 1,4-Dioxane by Methane Oxidizing Bacteria in Coupled Anaerobic-Aerobic Zones


Descriptive Note : Technical Report,01 Jun 2013,01 Feb 2016


Corporate Author : CB and I Federal Service, LLC. Lawrenceville United States


Personal Author(s) : Schaefer,Charles ; van Groos,Paul K ; Hatzinger,Paul


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1022557.pdf


Report Date : 11 Feb 2016


Pagination or Media Count : 43


Abstract : Microcosm tests, microbial enrichments, pure culture studies, and column experiments were performed to evaluate the co-metabolic biodegradation of 1,4-dioxane ( 1,4-D) by methane-, ethane- and propane-oxidizing bacteria. Results obtained during this limited scope study showed that methane-oxidizing bacteria were not effective for promoting the biodegradation of 1,4-D. For all conditions examined, including use of copper chelators, various nutrients, pure and enrichment cultures, and soil collected from 3 different sites, 1,4-D biodegradation was not observed with methane as a cosubstrate. However, ethane- and propane-oxidizing bacteria were shown to biodegrade 1,4-D. Michaelis-Menten kinetic parameters were determined for both 1,4-D and ethane during degradation by an enrichment culture. Regression of the model parameters Vmax and Km for 1,4-dioxane were 4.6 +/- 1.7 x 10(-5) mg/mg/cell/hand 0.23 +/- 0.07 mg/L, respectively; Vmax and Km for ethane were 1.8 +/- 0.6 x 10(-3) mg/mg/cell/hand 0.064 +/- 0.02 mg/L, respectively. Overall, the data suggest that ethane and propane are more effective cosubstrates than methane for stimulating 1,4-D biodegradation.


Descriptors :   Ethers , aquifers , methane , ethanes , kinetics , bioremediation , Dioxanes , bacteria , soil science , groundwater


Subject Categories : Solid Wastes Pollution and Control
      Microbiology
      Ecology


Distribution Statement : APPROVED FOR PUBLIC RELEASE