Accession Number : AD1019383


Title :   The Next Generation of Synthetic Biology Chassis: Moving Synthetic Biology from the Laboratory to the Field


Descriptive Note : OSTP Journal Article,01 Sep 2016,30 Sep 2016


Corporate Author : US Army Research Laboratory Adelphi United States


Personal Author(s) : Adams, Bryn L


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1019383.pdf


Report Date : 26 Sep 2016


Pagination or Media Count : 8


Abstract : Escherichia coli (E. coli) has played a pivotal role in the development of genetics and molecular biology as scientific fields. It is therefore not surprising that synthetic biology (SB) was built upon E. coli and continues to dominate the field. However, scientific capabilities have advanced from simple gene mutations to the insertion of rationally designed, complex synthetic circuits and creation of entirely synthetic genomes. The point is rapidly approaching where E. coli is no longer an adequate host for the increasingly sophisticated genetic designs of SB. It is time to develop the next generation of SB chassis; robust organisms that can provide the advanced physiology novel synthetic circuits will require to move SB from the laboratory into fieldable technologies. This can be accomplished by developing chassis-specific genetic toolkits that are as extensive as those for E. coli. However, the holy grail of SB would be the development of a universal toolkit that can be ported into any chassis.This viewpoint article underscores the need for new bacterial chassis, as well as discusses some of the important considerations in their selection. It also highlights a few examples of robust, tractable bacterial species that can meet the demands of tomorrows state-of-the-art in SB. Significant advances have been made in the first 15 years since this field hasemerged. However, the advances over the next 15 years will occur not in laboratory organisms, but in fieldable species where the potential of SB can be fully realized in game changing technology.


Descriptors :   synthetic biology , genetic engineering , cyanobacteria , molecular biology , metabolic engineering , gene expression , osmotic pressure , pseudomonas infections , ribosomes , receptor sites (physiology) , cells (biology) , Escherichia coli , genome


Distribution Statement : APPROVED FOR PUBLIC RELEASE