Accession Number : AD1018277

Title :   Immobilization of Organophosphorus Acid Anhydrolase Mutant Y212F on Silica Nanospheres

Descriptive Note : Technical Report,01 Aug 2014,31 Dec 2014

Corporate Author : Edgewood Chemical Biological Center Aberdeen Proving Ground United States

Personal Author(s) : Guelta,Mark A ; Harvey,Steven P ; Dixon,Melissa M ; Baldwin,Richard

Full Text :

Report Date : 01 Sep 2016

Pagination or Media Count : 22

Abstract : We have engineered mutants of the wild type organophosphorus acid anhydrolase (OPAA) for activity on G-type chemical nerve agents and observed increased activity on the most-toxic enantiomers. Enzyme stability might be enhanced by attachment of the OPAA to a silicon dioxide nanoparticle. Conjugation of our enzyme mutant was performed by personnel at nanoComposix (San Diego, CA), which is a producer of customized nanoparticles. After conjugation, the activity of the enzymeparticle combination on pinacolyl methyl phosphonofluoridate (GD, soman) was measured and compared with free enzyme. Activity measurements were made to compare a free and immobilized OPAA mutant on the G-agent stimulant diisopropyl fluorophosphates (DFP) and soman.

Descriptors :   Chemical warfare agents , Toxic agents , enzymes , nerve agents , Nanoparticles , g agents

Distribution Statement : APPROVED FOR PUBLIC RELEASE