Accession Number : AD1017049


Title :   Nanotechnology-Based Detection of Novel microRNAs for Early Diagnosis of Prostate Cancer


Descriptive Note : Technical Report,15 Jul 2015,14 Jul 2016


Corporate Author : University of Nebraska Medical Center Omaha United States


Personal Author(s) : Kshirsagar,Prakash


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1017049.pdf


Report Date : 01 Aug 2016


Pagination or Media Count : 18


Abstract : Prostate cancer (PCa) is most commonly diagnosed and second leading cause of death in the US men, representing 8% of all male cancer deaths. Currently, PCa is monitored and managed by prostate-specific antigen (PSA) screening combined with digital rectal examination (DRE). PSA screening had made a paradigm-shifting impact on the detection and management of PCa. However, these tests have limitations, because of low predictive value (25-37%) and high false positive results due to lack of sensitivity and specificity, resulting in significant overtreatment of PCa patient. Hence, there is an urgent need to develop novel, non-invasive and more accurate biomarkers in conjugation with highly sensitive detection platform for more precise diagnosis of PCa. Recently, an altered expression of miRNAs (miRs) have been observed in PCa and correlated them as a potential biomarker for PCa. In the present study, we are quantifying the expression level of deregulated miRNAs in mouse and human PCa tissues as well as serum samples using an advanced nanotechnology-based sensing nanoparticle probes (i.e. nanoprobes). Overall, the proposed studies will lead to the development of efficient technology for the accurate and early detection of differentially expressed miRNAs using sensitive Au-nanoprobes. This could enable us to develop early detection markers for PCa and differentiate between androgen independent and aggressive PCa. Recently, we have successfully synthesized and characterized fluorescently-labeled DNA-gold nanoparticle probes via bioconjugation. The result of fluorescence-based assay revealed that successful conjugation of 150-200 oligonucleotides per Au nanoparticle. In parallel, our qPCR analysis on prostate tissues excised from PTEN conditional knockout and wild-type littermate controls identified differential expression level of miRNAs. We also analyzed expression patterns of miRNAs (identified through meta-analysis of previously published reports) in mouse PCa tissue


Descriptors :   prostate cancer , Nanotechnology , diagnosis (medicine) , FLUORESCENCE , assaying , biological detection , antigens , Deoxyribonucleic acids , Ribonucleic acidS , metallic nanoparticles , surface plasmon resonance , transmission electron microscopy , amines , biological markers , neoplasms , gel electrophoresis , TISSUES (BIOLOGY)


Distribution Statement : APPROVED FOR PUBLIC RELEASE