Accession Number : AD1016853


Title :   Novel Mechanism for Reducing Acute and Chronic Neurodegeneration After Traumatic Brain Injury


Descriptive Note : Technical Report,01 Jul 2015,30 Jun 2016


Corporate Author : University of Calfornia, Davis Davis United States


Personal Author(s) : Lyeth,Bruce


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1016853.pdf


Report Date : 01 Jul 2016


Pagination or Media Count : 19


Abstract : Purpose: The purpose of this project is to develop a radically different strategy to reduce brain glutamate excitotoxicity and treat TBI. We will supplement a natural blood-resident enzymatic system with glutamate-oxaloacetate transaminase (rGOT) and the co-substrate oxalo-acetate (OxAc) with the objective of reducing blood levels of glutamate. This will produce a brain-to-blood gradient of glutamate which will enhance the removal of excess glutamate from the brain. Scope: We will test this novel and powerful neuroprotective treatment in a rat model of repetitive mild (concussive) TBIs and in a model of a single moderate TBI. Major Findings: We have: 1.Troubleshooting and refinement of blood serum glutamate assays to reduce variability. 2. Completed statistical analysis of behavioral experiments examining effects of rGOT and rGOT + OxAc on outcome on rotarod and Morris watermaze. 3. Measured time course of GOT levels in blood and levels after iv injection of 130ug/kg of rGOT. 4.Completed sectioning of brain tissue and completed 60% of hippocampal neuronal cell counting. 5.Troubleshooting and refinement of CSF extraction resulting in reliable measurement of glutamate in CSF.


Descriptors :   BRAIN INJURIES , NEUROTRANSMITTERS , GLUTAMATES , enzymes , BLOOD VOLUME , cognition , NEURONS , behavior , cells (biology) , statistical analysis , therapeutics , SYMPTOMS AND GENERAL PATHOLOGY , biomedical research , CONCUSSION


Distribution Statement : APPROVED FOR PUBLIC RELEASE