Accession Number : AD1016117

Title :   Electroporation of Mammalian Cells by Nanosecond Electric Field Oscillations and its Inhibition by the Electric Field Reversal

Descriptive Note : Journal Article

Corporate Author : 711 HPW/RHDR JBSA Fort Sam Houston United States

Personal Author(s) : Gianulis,Elena C ; Lee,Jimo ; Jiang,Chunqi ; Xiao,Shu ; Ibey,Bennet L ; Pakhomov,Andrei G

Full Text :

Report Date : 08 Sep 2015

Pagination or Media Count : 11

Abstract : The study compared electroporation efficiency of bipolar and unipolar nanosecond electric field oscillations (NEFO). BipolarNEFO was a damped sine wave with 140 ns first phase duration at 50 height; the peak amplitude of phases 2-4 decreased to 35 ,12 , and 7 of the first phase. This waveform was rectified to produce unipolar NEFO by cutting off phases 2 and 4. Membranepermeabilization was quantified in CHO and GH3 cells by uptake of a membrane integrity marker dye YO-PRO-1 (YP) and by themembrane conductance increase measured by patch clamp. For treatments with 1-20 unipolar NEFO, at 9.6-24 kV/cm, 10 or 20 Hz,the rate and amount of YP uptake were consistently 2-3-fold higher than after bipolar NEFO treatments, despite delivering lessenergy. However, the threshold amplitude was about 7 kV/cm for both NEFO waveforms. A single 14.4 kV/cm unipolar NEFOcaused a 1.5-2 times greater increase in membrane conductance (p0.05) than bipolar NEFO, along with a longer and less frequentrecovery. The lower efficiency of bipolar NEFO was preserved in Ca2 -free conditions and thus cannot be explained by the reversalof electrophoretic flows of Ca2 . Instead, the data indicate that the electric field polarity reversals reduced the pore yield.

Distribution Statement : APPROVED FOR PUBLIC RELEASE