Accession Number : AD1014066


Title :   The Role of the Transcription Factors MtrR and MtrA in the Fitness of the Pathogen Neisseria gonorrhoeae


Descriptive Note : Technical Report


Corporate Author : Uniformed Services University Of The Health Sciences Bethesda United States


Personal Author(s) : Warner,Douglas M


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1014066.pdf


Report Date : 19 Oct 2007


Pagination or Media Count : 178


Abstract : Antibiotic resistance has been a health burden for humankind since the inception of antibiotic therapy. Generally, antibiotic resistance levies a fitness toll on the bacterium; however, previous work showed that the MtrC-MtrD-MtrE antibiotic efflux pump of the pathogen Neisseria gonorrhoeae is important for bacterial colonization of the murine lower genital tract in the absence of antibiotic substrates. The mtrCDE operon is under the control of MtrR, a repressor protein which, when mutated, increases the level of resistance to MtrC-MtrD-MtrE substrates. Results here show that mutation of mtrR confers an in vivo fitness advantage to N. gonorrhoeae via derepression of the mtrCDE operon. Conversely, when MtrA, the transcriptional activator of mtrCDE, is mutated MtrA-deficient strains are attenuated in vivo.


Descriptors :   Neisseria gonorrhoeae , ANTIBIOTICS , DRUG TOLERANCE , pathogenesis , therapy , bacteria , colonies (biology) , mutations , proteins , receptor sites (physiology) , transcription (genetics)


Distribution Statement : APPROVED FOR PUBLIC RELEASE