Accession Number : AD1013280


Title :   Regulation of CD4+ T-Cell Function by Membrane Cholesterol


Descriptive Note : Technical Report


Corporate Author : Uniformed Services University Of The Health Sciences Bethesda United States


Personal Author(s) : Surls,Jacqueline D


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1013280.pdf


Report Date : 13 Mar 2012


Pagination or Media Count : 158


Abstract : The CD4 T helper cells play a central role in initiating immune responses to various types of infections that have breached the immune defense. In the context of vaccination, CD4 T-cells are critical for establishing broad, long-lasting protective immunity. It is known that T-regulatory cells (T-regs) can limit CD4 T-cell responses during influenza viral infection, however less is known about the effect of T-regs in influenza vaccination. In this dissertation, I first present evidence that the size of CD4+Foxp3+ T-reg pool is an important modulatory component of the primary and memory T-cell responses to influenza vaccination. Herein, I found that immunization of BALB/c mice with a prototype of influenza A/PR/8/34 virus vaccine expanded the CD4+Foxp3+ T-reg pool and fostered the development of virus-specific CD4+Foxp3+ T-reg cells. Increasing the size of Foxp3+ T-reg pool did not alter the primary PR8-specific B-cell response, but it did suppress the primary and memory PR8-specific T helper responses induced by vaccination. In contrast, the vaccination-induced T helper cell response was augmented when the pool of CD4+Foxp3+ T-regs was decreased by 50%.


Descriptors :   molecular biology , WHITE BLOOD CELLS , infectious diseases , immunity , response (biology) , INFLUENZA VACCINES , MEMBRANES BIOLOGY , CHOLESTEROL , mice , ANTIGEN ANTIBODY REACTIONS , Receptor sites (Physiology) , biosynthesis , CYTOKINES


Distribution Statement : APPROVED FOR PUBLIC RELEASE