Accession Number : AD1013022


Title :   Evaluating Vaccine Candidates for Filariasis


Descriptive Note : Technical Report


Corporate Author : Uniformed Services University Of The Health Sciences Bethesda United States


Personal Author(s) : Morris,Christopher P


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1013022.pdf


Report Date : 16 Apr 2013


Pagination or Media Count : 153


Abstract : Parasites of the family Onchocercidae, commonly referred to as filarial worms, can cause severely devastating conditions such as elephantiasis and blindness. While there are programs currently in place to try to control or eradicate these diseases through mass drug administration, there are no vaccines available to prevent infection with these pathogens. There has been an interest in vaccination with hidden antigens, antigens to which the host does not mount an adaptive immune response during infection, in helminths. We found that the soluble fraction of the intestine (GutAg) of L. sigmodontis exhibits some hidden qualities, in that IgG antibody titers to these antigens are much lower than to the soluble fraction of the whole worm (LsAg), and that cytokine production by splenocytes is lower in response to GutAg than to LsAg. However, immune responses associated with allergy, including IgE titers and Basophil activation, were similar in response to LsAg and GutAg. Furthermore, vaccination with GutAg and CpG/Alum was not protective against challenge infection. Similarly, a vaccination protocol using LsAg and CpG/alum did not protect against challenge infection. However, vaccination with irradiated larvae results in around 80% protection, yet this method of vaccination is not practical in humans. We hypothesized that comparing the immune responses in the irradiated larval vaccinated mice compared to the LsAg vaccinated mice may provide some insight into future methods of vaccination. We found that while vaccination with irradiated larvae (iL3) and LsAg with CpG/Alum induced similar IFN- production in response to LsAg, IL-4 production was much higher in the iL3 vaccinated mice. Vaccination with LsAg adsorbed to alum induced strong IL-4 responses to LsAg, but again did not result in a protective immune response.


Descriptors :   parasites , elephantiasis , blindness , vaccines , infectious diseases , antigens , response(biology) , larvae , antibodies , proteins , proteomics


Distribution Statement : APPROVED FOR PUBLIC RELEASE