Accession Number : AD1012363


Title :   A Thapsigargin-Resistant Intracellular Calcium Sequestering Compartment in Rat Brain


Descriptive Note : Technical Report


Corporate Author : Uniformed Services University Of The Health Sciences Bethesda United States


Personal Author(s) : Watson,William D


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1012363.pdf


Report Date : 31 Mar 2000


Pagination or Media Count : 143


Abstract : Calcium plays a central regulatory role in the normal function of all cells. Electrical, secretory, and metabolic activities of cells in the brain require fine control over ionized cytoplasmic calcium levels. Intracellular calcium levels are controlled by a diverse set of cytoplasmic and membrane-associated mechanisms including calcium binding proteins. channels, pumps, and exchangers. The endoplasmic reticulum (ER) calcium stores have a major impact on neuronal intracellular signaling. Most of the ER in neurons and glia appears to accumulate calcium by energy driven ion pumps known as sarco/endoplasmic reticulum calcium ATPases (SERCAs), which are potently and selectively inhibited by thapsigargin. However, the ER represents a heterogeneous network of cisternae in which calcium-accumulating subcompartments may be spatially and functionally distinct. We describe here the characterization of a novel calcium accumulating subcompartment of rat brain ER, which is insensitive to thapsigargin. This compartment accumulates calcium in a magnesium and ATP-dependent manner and is distinguished from thapsigargin-sensitive calcium pools with respect to anion permeability, inhibitor sensitivity, sensitivity to calcium mobilizers, and brain anatomical distribution.


Descriptors :   rats , brains , calcium , endoplasmic reticulum , cell physiological processes , Statistical Analysis , temperature , inhibitors , inhibition


Subject Categories : Anatomy and Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE