Accession Number : AD1012133


Title :   Highly Resolved Sub-Terahertz Vibrational Spectroscopy of Biological Macromolecules and Bacteria Cells


Descriptive Note : Technical Report,01 Mar 2014,01 Mar 2015


Corporate Author : Edgewood Chemical Biological Center Aberdeen Proving Ground United States


Personal Author(s) : Maswadeh,Waleed ; Vanderbeek,Richard ; Moon,Raphael ; Tripathi,Ashish ; Moyer,Aaron M ; Globus,Tatiana ; Gelmont,Boris ; Sizov,Igor ; Ferrance,Jerome


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1012133.pdf


Report Date : 01 Jul 2016


Pagination or Media Count : 54


Abstract : Sub-terahertz (sub-THz) vibrational spectroscopy for biodetection is based on specific resonance features and vibrational modes or groups of modes at close frequencies that are in the absorption (transmission) spectra of large biological molecules and intact bacterial cells and spores. Improvements in sensitivity, especially in the discriminative capability of sub-THz vibrational spectroscopy for detection, characterization, and identification of bacterial organisms, require higher spectral resolution to resolve the spectral features. Herein we describe a new, continuous-wave and frequency-domain spectroscopy sensor with imaging capability. This spectroscopy system was operated at room temperature in the sub-THz spectral region between 315 and 480 GHz. We present experimental spectra obtained from biological macromolecules and species using this spectrometer, and we compare biological spectra with simulation results using molecular dynamics. Observed, multiple, intense and specific resonances in transmission and absorption spectra from nanogram samples with spectral line widths as small as 0.11 cm provide the conditions for determining reliable discriminative capability and for monitoring interactions between biomaterials and reagents in near real time.


Descriptors :   MOLECULAR DYNAMICS SIMULATIONS , CELLS(BIOLOGY) , BIOLOGICAL DETECTION , BIOSENSORS , VIBRATIONAL SPECTRA , SPECTROSCOPY , TERAHERTZ RADIATION , CONTINUOUS WAVES , FREQUENCY DOMAIN , Insulin , MACROMOLECULES , BACTERIA


Distribution Statement : APPROVED FOR PUBLIC RELEASE