Accession Number : AD1011821

Title :   Hypoxia Inducible Factor 1 (HIF1) Activation in U87 Glioma Cells Involves a Decrease in Reactive Oxygen Species Production and Protein Kinase C Activity

Descriptive Note : Technical Report

Corporate Author : Uniformed Services University of The Health Sciences Bethesda United States

Personal Author(s) : Forbes,Robert A

Full Text :

Report Date : 29 Jun 1998

Pagination or Media Count : 138

Abstract : Hypoxia regulates physiological functions including erythropoeisis, ventillatory drive, angiogenesis, vascular tone, and glycolytic function - all which are essential for systemic and cellular adaptation to lowered oxygen tension. This is mediated in part through induction of a hypoxia inducible transcription factor (HIF-l) which is instrumental in the regulation of genes such as vascular endothelial growth factor (VEGF) and erythropoietin (EPa). The purpose of the following work was to identify specific elements of the hypoxic signaling pathways involved in HIF-l activation in a glial derived cell line (U87 glioma) using gel shift analysis. Since lowering of available oxygen effectively lowers the production of reactive oxygen species (ROS), this shift in ROS production could be the hypoxic signal which mediated HIF-l induction.

Descriptors :   HYPOXIA

Distribution Statement : APPROVED FOR PUBLIC RELEASE