Accession Number : AD1011468


Title :   The Expression of Fos, Jun and AP-1 DNA Binding Activity in Rat Supraoptic Nucleus Neurons Following Acute Versus Repeated Osmotic Stimulation


Descriptive Note : Technical Report


Corporate Author : Uniformed Services University Of The Health Sciences Bethesda United States


Personal Author(s) : Wang,Ke


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1011468.pdf


Report Date : 22 Jun 1995


Pagination or Media Count : 176


Abstract : FOB and Jun are protein products of their respective proto-oncogenes, c-fos and c-jun. Nuclear concentrations of both proteins rapidly, but transiently, rise after thepresentation of a variety of stimuli under in vitro and in vivo conditions. Fos and Jun can form heterodimeric proteins that act as transcription factors to regulate late response genes.Previous work has demonstrated that hypertonic saline injection results in Fos expression in supraoptic nucleus (SON) neurons that mediate body water homeostasis. Since Fos is thought to act mostly in conjunction with Jun, I first developed a double-label fluorescence immunocytochemical procedure that permits simultaneous visualization of neurons expressing Fos and Jun. After hypertonic saline administration, the immunostaining of c-Fos and c-Jun was coexistent in the majority of rnagnocellular neurons of SON.


Descriptors :   proteins , genes , Hybridization , saline solution , deoxyribonucleic acids , Stimulation (Physiology) , gene expression , Nervous system , Transcription (Genetics) , Hypothalamus , rats , Cytochemistry , immunochemistry , Ribonucleic acids , Plasmids


Distribution Statement : APPROVED FOR PUBLIC RELEASE