Accession Number : AD1011348

Title :   Detection of siRNA Mediated Target mRNA Cleavage Activities in Human Cells by a Novel Stem-Loop Array RT-PCR Analysis

Descriptive Note : Journal Article

Corporate Author : The University of Texas MD Anderson Cancer Center Houston United States

Personal Author(s) : Xu,Kai ; Roth,Jack A ; Ji,Lin ; Lin,Jing

Full Text :

Report Date : 07 Sep 2016

Pagination or Media Count : 8

Abstract : The small interfering RNA (siRNA)-mediated target mRNA cleavage activity generates cleaved mRNA fragments with varied termini, which creates major technical challenges for the accurate and efficient detection and verification of cleavage sites on target mRNAs. Here we used a sensitive stem-loop array reverse transcription polymerase chain reaction (SLA-RT-PCR) approach to detect and verify the siRNA-mediated target mRNA cleavage sites by determining precise sequences at the 3'- termini of cleaved mRNA fragments in human cells under physiological conditions. Our results demonstrated the great potential and broad applications of using the SLA-RT-PCR as a sensitive, cost-efficient, and high-throughput tool to systematically detect siRNA-targeted mRNA cleavage sites and fragments in human cells.

Descriptors :   cleavage , Ribonucleic acids , electrophoresis , detection , sensitivity , sequences , targets

Distribution Statement : APPROVED FOR PUBLIC RELEASE