Accession Number : AD1011140


Title :   Archive of Samples for Long-Term Preservation of RNA and Other Nucleic Acids


Descriptive Note : SBIR Report,01 Sep 2015,15 May 2016


Corporate Author : Biomatrica San Diego United States


Personal Author(s) : Dodson, Gerald ; Liberal,Vasco


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1011140.pdf


Report Date : 15 May 2016


Pagination or Media Count : 50


Abstract : The project's objective was the development of a scalable all ambient temperature biological sample workflow preserving nucleic acids for molecular analysis. This ambient workflow can be developed for large scale blood collection, transport, and long-term archiving without the use of costly and unreliable cold chain management, using commercially available biopreservation products. For the development of the automated workflow we have chosen Biomatrica's commercially validated products RNAgard Blood tubes, RNAstable and DNAstable Plus, an off the shelf liquid handler (Beckman), acommercially available dryer (Genevac Inc) and small 384 well storage tubes with a commercial tube sealing system for compact storage (Thermo). In this research period we have achieved the final Phase II milestone of processing 500 samples in a week.


Descriptors :   NUCLEIC ACIDS , CHEMICAL STABILITY , STORAGE , automation


Distribution Statement : APPROVED FOR PUBLIC RELEASE