Accession Number : AD1010886


Title :   Toxin MqsR Cleaves Single-Stranded mRNA with Various 5 Ends


Descriptive Note : Journal Article


Corporate Author : Pennsylvania State University University Park United States


Personal Author(s) : Chowdhury,Nityananda ; Kwan,Brian W ; McGibbon,Louise C ; Babitzke,Paul ; Wood,Thomas K


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/1010886.pdf


Report Date : 24 Aug 2016


Pagination or Media Count : 8


Abstract : Toxin/antitoxin (TA) systems are the means by which bacterial cells become persistent; that is, those cells that are tolerant to multiple environmental stresses such as antibiotics by becoming metabolically dormant. These persister cells are responsible for recalcitrant infections. Once toxins are activated by the inactivation of antitoxins (e.g., stress-triggered Lon degradation of the antitoxin), many toxins reduce metabolism by inhibiting translation (e.g., cleaving mRNA, reducing ATP). The MqsR/MqsA TA system of Escherichia coli cleaves mRNA to help the cell withstand oxidative and bile acid stress. Here, we investigated the role of secondary structure and 5 mRNA processing on MqsR degradation of mRNA and found that MqsR cleaves only single-stranded RNA at 5-GCU sites and that MqsR is equally active against RNA with 5-triphosphate, 5-monophosphate, and 5-hydroxylgroups.


Descriptors :   molecular biology , Toxins and antitoxins , Ribonucleic acids , cleavage , infection , sequences , stress (physiology) , substrates , bacteria , cells(biology) , genes


Distribution Statement : APPROVED FOR PUBLIC RELEASE